Cofiler user manual

Controlled versions of Department of Forensic Biology Manuals only exist electronically on the OCME intranet. All printed versions are non-controlled copies. Forensic Biology Case Management Manual Approval Form COfiler* D3S, D16S, Amelogenin, TH01, TPOX, CSF1PO, D7S Yes Profiler Plus* D3S, VWA, FGA, Amelogenin, D8S or from the kit manuals in the cases of Penta D, Penta E, and D2S GenBank GenBank Allele Allele Product Size MiniSTR Size STR Locus Accession Allele Range Spread (STR Kit) Size Reduction CSF1PO X 12 6–16 40 bp – bp (Cofiler) 89– bp bp FGA M 21 – bp – bp (ProPlus) – bp 71 bp. reduce spurious amplification. See (ProfilerPlus, Cofiler, SGMplus manuals, Applied Biosystems, Foster City, CA; PowerPlex kits, Promega, Madison, WI; Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than pg of DNA. Forensic Sci Intl , ). Step 3.

ABI Profiler Plus / Cofiler Users Manuals. Allele Frequencies for Fourteen STR Loci of the PowerPlex and Multiplex Systems and Penta D Locus In Caucasians, African-Americans, Hispanics, and Other Populations of the United States of America and Brazil, Levedakou www.doorway.ru, JFS, , 46(3) For each sample, 13 loci were amplified simultaneously using AmpF/STR Profiler Plus and AmpF/STR COfiler PCR Amplification Kits (Applied Biosystems, Foster City, CA, USA) at the D3S, vWA, FGA. Contents i 1 Introduction Short Tandem Repeat Loci.

The Identifiler kit is unable to amplify a blood sample from a leather glove. With its enhanced formulation, the Identifiler Plus kit helps deliver a full profile. For both kits, 1 ng of input DNA and 28 cycles of amplification were used. Helps overcome PCR inhibition through the use of a high performance PCR buffer system and optimized thermal. 6 AmpFlSTR® COfiler® PCR Amplification Kit User Bulletin: Kit Overview, Experiments, and Results Kit Overview, Developmental Validation, and Performance Validation Materials and equipment Materials and equipment Kit contents and storage The COfiler® Kit (Part no. ) contains materials sufficient to perform. COfiler™ Kit Template v4, AmpFLSTR® Profiler Plus™ Kit Template v4, AmpFLSTR® Profiler™ Kit Template v3, AmpFLSTR® Green™ I™ Kit Template v3, and AmpFLSTR® Blue™ Kit Template v3 require Genotyper® Software v or higher. • The Kazam macros within a color have been rearranged so that the steps for.